HALO columns are designed for hyper-fast HPLC separations
Column: HALO C18, 4.6 x 50 mm Mobile Phase: 55%
Methanol, 45% 25mM sodium phosphate, pH 2.5 Flow Rate: 2.2 ml/min
Temperature: ambient (24 °C) Pressure: 4,700 psi, 320 bar
HALO columns are designed for hyper-fast separations so that higher sample throughput can be achieved. In this example, the HALO column separated seven compounds in less than 48 seconds with better than baseline resolution for all peak pairs.
HALO columns are designed for hyper-fast separations so that higher sample throughput can be achieved. In this example, the HALO column separated seven compounds in less than 48 seconds with better than baseline resolution for all peak pairs.
Stability testing of a HALO
HPLC column
Column: HALO C8, 2.1 x 50 mm Mobile Phase: 50%
Acetonitrile, 50% water Flow Rate: 1.0 ml/min Temperature: ambient
(24 °C) Pressure: 3,800 psi, 260 bar
A HALO column was run under high flow conditions to test bed stability. After 500 sample injections and over 40,000 column volumes, there was no evidence of any change to the packing bed.
A HALO column was run under high flow conditions to test bed stability. After 500 sample injections and over 40,000 column volumes, there was no evidence of any change to the packing bed.
Comparison of van Deemter plots
Van Deemter plots are a convenient way to compare
the efficiency of HPLC columns. In this comparison we see that HALO
columns are more efficient than columns packed with 5 μm or 3.5 μm
particles and that they can be run at higher mobile phase linear velocity
and still maintain their resolving power.
Pittcon 2007 presentation by Dr. Joseph Kirkland – New Fused-Core
Particles for Very Fast HPLC Separations
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